Development a New Human Skin Continuous Cell Line Sensitive to Mumps Virus
نویسندگان
چکیده مقاله:
Background and Aims: During the last years a new Foreskin cell line strain (RFSC) has been isolated, characterized and stored in a representative cell bank stock in liquid nitrogen as a part of HCB (Human Cell Bank) development program in Razi institute. Materials and Methods: The cell population was serially passaged for mass production purpose based on WHO schedule .The controls on pool samples were carried out upon our sampling method and international recommendations. Results: The data concerning parameters as well as the result of compulsory controls on Kario logy oncogenicity, cross contaminations, species specificity, no oncogenicity, and mycoplasma free of a human cell line that contained heteroploid chromosomal characterizations are performed. Conclusion: Kariological examination found an obvious discrepancy in the frequency of aberrations due to high passage level that produced a heteroploid continuous cell line. Virus susceptibility shows RFSC-1 more sensitive to RS#12 Mumps virus strain. This cell line is completely sterile and has no any cross contamination to other pathogen and can be used for production and control of Mumps vaccine.
منابع مشابه
development a new human skin continuous cell line sensitive to mumps virus
background and aims: during the last years a new foreskin cell line strain (rfsc) has been isolated, characterized and stored in a representative cell bank stock in liquid nitrogen as a part of hcb (human cell bank) development program in razi institute. materials and methods: the cell population was serially passaged for mass production purpose based on who schedule .the controls on pool sampl...
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عنوان ژورنال
دوره 7 شماره None
صفحات 7- 13
تاریخ انتشار 2013-11
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